Epigallocatechin gallate restores the reduction of protein phosphatase 2 A subunit B caused by middle cerebral artery occlusion / 한국실험동물학회지

Background@#Epigallocatechin gallate (EGCG) is a flavonoid compound commonly found in green tea. It exhibits antioxidant, anti-inflammatory, and neuroprotective effects in cerebral ischemia. Protein phosphatase 2 A (PP2A) is an important serine/threonine phosphatase enzyme involved in various cellular activities. PP2A subunit B is present abundantly in the brain and plays an important role in the nervous system. We investigated the effect of EGCG on the expression level of PP2A subunit B in cerebral ischemia caused by middle cerebral artery occlusion (MCAO). EGCG (50 mg/kg) or vehicle was injected into the peritoneal cavity prior to MCAO surgery. Neurological behavior tests were performed 24 h after MCAO, and right cerebral cortex tissue was collected. Cerebral ischemia caused serious neurological abnormalities, which were alleviated by EGCG administration. We screened the expression of PP2A subunits containing A, B, and C using reverse-transcription PCR. We confirmed that PP2A subunit B exhibited significant changes in MCAO animals compared to subunits A and C. We continuously examined the expression of PP2A subunit B protein in MCAO animals using Western blot analysis. @*Results@#EGCG alleviated the reduction of PP2A subunit B protein by MCAO damage. In addition, immunohistochemistry demonstrated a decrease in the number of PP2A subunit B-positive cells in the cerebral cortex, and EGCG attenuated this decrease. Maintenance of PP2A subunit B is important for normal brain function. @*Conclusion@#Therefore, our findings suggest that EGCG exerts neuroprotective effects against cerebral ischemia through modulation of PP2A subunit B expression.

Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach / 한국실험동물학회지

Background@#Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals. @*Results@#We found that chlorogenic acid alleviated in changes in adenosylhomocysteinase, glycerol-3-phosphate dehydrogenase, eukaryotic translation initiation factor 4A-II, apolipoprotein A-I, and mu-crystallin. These proteins were reduced in MCAO animals with vehicle, and these reductions were attenuated by chlorogenic acid treatment. The mitigation of this reduction by chlorogenic acid was confirmed by the reverse transcription PCR technique. These proteins are associated with energy metabolism, protein synthesis, inflammation, and physiological metabolism. They are involved in the neuroprotective effect of chlorogenic acid. These results showed that chlorogenic acid alleviates the neurological disorders caused by MCAO and regulates the expression of proteins involved in neuroprotection. @*Conclusions@#Therefore, our findings provide evidence that chlorogenic acid plays a neuroprotective role in stroke animal models by controlling specific proteins.

Alleviation of γ-enolase decrease by the chlorogenic acid administration in the stroke animal model

Stroke is a major cause of death and long-term disability. Chlorogenic acid is a phenolic compound with a potent neuroprotective effect. γ-enolase is a phosphopyruvate hydratase found in mature neurons and plays an important role in the neuronal survival. This study investigated whether chlorogenic acid regulates the expression of γ-enolase during cerebral ischemia. Middle cerebral artery occlusion (MCAO) was performed to indcue cerebral ischemia. Adult male rats were used and chlorogenic acid (30 mg/kg) or phosphate buffered saline (PBS) was injected intraperitoneally 2 hours after MCAO surgery. Cerebral cortical tissues were collected 24 hours after MCAO surgery. Our proteomic approach identified the reduction of γ-enolase caused by MCAO damage and the mitigation of this reduction by chlorogenic acid treatment. Results of reverse transcription-polymerase chain reaction and Western blot analyses showed decrease in γ-enolase expression in PBS-treated MCAO group. However, chlorogenic acid treatment attenuated this decrease. Results of immunofluorescence staining showed the change of γ-enolase by chlorogenic acid treatment. These results demonstrated that chlorogenic acid regulates the γ-enolase expression during MCAO-induced ischemia. Therefore, we suggest that chlorogenic acid mediates the neuroprotective function by regulating the γ-enolase expression in cerebral ischemia and may be used as a therapeutic agent for brain diseases including stroke.

Chlorogenic acid modulates the ubiquitin– proteasome system in stroke animal model / 한국실험동물학회지

Background@#Chlorogenic acid, a phenolic compound, has potent antioxidant and neuroprotective properties. The ubiquitin–proteasome system is an important regulators of neurodevelopment and modulators of neuronal function. This system is associated with neurodevelopment and neurotransmission through degradation and removal of dam‑ aged proteins. Activation of the ubiquitin–proteasome system is a critical factor in preventing cell death. We have pre‑ viously reported a decrease in the activity of the ubiquitin–proteasome system during cerebral ischemia. This study investigated whether chlorogenic acid regulates the ubiquitin–proteasome system in an animal stroke model. In adult rats, middle cerebral artery occlusion (MCAO) surgery was performed to induce focal cerebral ischemia. Chlorogenic acid (30 mg/kg) or normal saline was injected into the abdominal cavity 2 h after MCAO surgery, and cerebral cortex tissues were collected 24 h after MCAO damage. @*Results@#Chlorogenic acid attenuated neurobehavioral disorders and histopathological changes caused by MCAO damage. We identified the decreases in ubiquitin C-terminal hydrolase L1, ubiquitin thioesterase OTUB1, proteasome subunit α type 1, proteasome subunit α type 3, and proteasome subunit β type 4 expression using a proteomics approach in MCAO animals. The decrease in these proteins was alleviated by chlorogenic acid. In addition, the results of reverse transcription-polymerase chain reaction confirmed these changes. The identified proteins were markedly reduced in MCAO damage, while chlorogenic acid prevented these reductions induced by MCAO. The decrease of ubiquitin–proteasome system proteins in ischemic damage was associated with neuronal apoptosis. @*Conclusions@#Our results showed that chlorogenic acid regulates ubiquitin–proteasome system proteins and pro‑ tects cortical neurons from neuronal damage. These results provide evidence that chlorogenic acid has neuroprotec‑ tive effects and maintains the ubiquitin–proteasome system in ischemic brain injury.

Retinoic acid regulates the ubiquitin– proteasome system in a middle cerebral artery occlusion animal model / 한국실험동물학회지

Background@#Retinoic acid is a major metabolite of vitamin A and exerts beneficial effects including anti-oxidant and anti-inflammatory activities in neurons. The ubiquitin–proteasome system is an important biological system that regulates cell survival. Ubiquitination regulates protein degradation and plays an important role in oxidative stress. Deubiquitinating enzymes cleave ubiquitin from proteins and control ubiquitination-induced degradation. We detected decreases in ubiquitin carboxy-terminal hydrolase L1, ubiquitin thioesterase OTUB1, and proteasome subunit alpha types 1 and 3 in cerebral ischemic damage. In this study, we investigated whether retinoic acid regulates the expression of deubiquitinating enzymes ubiquitin carboxy-terminal hydrolase L1, ubiquitin thioesterase OTUB1, and proteasome subunit alpha types 1 and 3 in cerebral ischemic injury. Right middle cerebral artery occlusion (MCAO) was performed to induce cerebral ischemic damage in male rats. Retinoic acid (5 mg/kg) or vehicle was intraperitoneally injected every day from 4 days before surgery. Neurological behavioral tests were performed 24 h after MCAO, and right cerebral cortical tissues were collected. @*Results@#MCAO damage caused neurological behavioral dysfunction, and retinoic acid alleviated these deficits. The identified proteins decreased in MCAO animals with vehicle, while retinoic acid treatment attenuated these decreases.The results of proteomic study were confirmed by a reverse transcription-PCR technique. Expressions of ubiquitin carboxy-terminal hydrolase L1, ubiquitin thioesterase OTUB1, and proteasome subunit alpha types 1 and 3 were decreased in MCAO animals treated with vehicle. Retinoic acid treatment alleviated these MCAO-induced reductions. The ubiquitin–proteasome system plays an essential role in maintaining cell function and preserving cell shape against ischemic damage. @*Conclusions@#These findings suggest that retinoic acid regulates ubiquitin- and proteasome-related proteins including ubiquitin carboxy-terminal hydrolase L1, ubiquitin thioesterase OTUB1, and proteasome subunit alpha types 1 and 3 in a brain ischemia model. Changes in these proteins are involved in the neuroprotective effects of retinoic acid.

Quercetin ameliorates glutamate toxicity-induced neuronal cell death by controlling calcium-binding protein parvalbumin

Background@#Glutamate is the main excitatory neurotransmitter. Excessive glutamate causes excitatory toxicity and increases intracellular calcium, leading to neuronal death. Parvalbumin is a calcium-binding protein that regulates calcium homeostasis. Quercetin is a polyphenol found in plant and has neuroprotective effects against neurodegenerative diseases. @*Objectives@#We investigated whether quercetin regulates apoptosis by modulating parvalbumin expression in glutamate induced neuronal damage. @*Methods@#Glutamate was treated in hippocampal-derived cell line, and quercetin or vehicle was treated 1 h before glutamate exposure. Cells were collected for experimental procedure 24 h after glutamate treatment and intracellular calcium concentration and parvalbumin expression were examined. Parvalbumin small interfering RNA (siRNA) transfection was performed to detect the relation between parvalbumin and apoptosis. @*Results@#Glutamate reduced cell viability and increased intracellular calcium concentration, while quercetin preserved calcium concentration and neuronal damage. Moreover, glutamate reduced parvalbumin expression and quercetin alleviated this reduction. Glutamate increased caspase-3 expression, and quercetin attenuated this increase in both parvalbumin siRNA transfected and non-transfected cells. The alleviative effect of quercetin was statistically significant in non-transfected cells. Moreover, glutamate decreased bcl-2 and increased bax expressions, while quercetin alleviated these changes. The alleviative effect of quercetin in bcl-2 family protein expression was more remarkable in non-transfected cells. @*Conclusions@#These results demonstrate that parvalbumin contributes to the maintainace of intracellular calcium concentration and the prevention of apoptosis, and quercetin modulates parvalbumin expression in glutamate-exposed cells. Thus, these findings suggest that quercetin performs neuroprotective function against glutamate toxicity by regulating parvalbumin expression.

Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice / 한국실험동물학회지

Baicalin is a natural flavonoid that exerts a variety of pharmaceutical effects such as anti-inflammatory and antioxidant. Lipopolysaccharide (LPS) is an endotoxin that releases inflammatory cytokines and induces inflammatory response. This study was investigated the anti-inflammatory mechanism of baicalin against LPS-induced inflammatory response in the hippocampus. Adult mice were randomly grouped into control, LPS-treated, and LPS and baicalin co-treated animals. LPS (250 μg/kg/day) and baicalin (10 mg/kg/day) were administered intraperitoneally for 7 consecutive days. We measured neuroglia cells activation and inflammatory factors activation using Western blot analysis and immunofluorescence staining techniques. Ionized calcium binding adaptor molecule-1 (Iba-1) and glial fibrillary acidic protein (GFAP) are widely used as microglia and astrocyte markers, respectively. LPS treatment increased Iba-1 and GFAP expression, while baicalin co-treatment attenuated this overexpression. Nuclear factor-kappa B (NF-κB) is a key mediator of inflammation. Baicalin co-treatment alleviated LPS-induced increase of NF-κB in the hippocampus. In addition, LPS treatment upregulated pro-inflammatory cytokines including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). However, baicalin co-treatment prevented LPS-induced increases of IL-1β and TNF-α in the hippocampus. Results from the present study showed that baicalin suppresses LPS-induced neuroinflammation by regulating microglia and astrocyte activation and modulating inflammatory factors in the hippocampus. Thus, these results demonstrate that baicalin has neuroprotective effect by alleviates microglia and astrocyte activation and modulates inflammatory response by suppressing NF-κB expression in hippocampus with neuroinflammation caused by LPS.

Lipopolysaccharide induces neuroglia activation and NF-κB activation in cerebral cortex of adult mice / 한국실험동물학회지

Lipopolysaccharide (LPS) acts as an endotoxin, releases inflammatory cytokines, and promotes an inflammatory response in various tissues. This study investigated whether LPS modulates neuroglia activation and nuclear factor kappa B (NF-κB)-mediated inflammatory factors in the cerebral cortex. Adult male mice were divided into control animals and LPS-treated animals. The mice received LPS (250 µg/kg) or vehicle via an intraperitoneal injection for 5 days. We confirmed a reduction of body weight in LPS-treated animals and observed severe histopathological changes in the cerebral cortex. Moreover, we elucidated increases of reactive oxygen species and oxidative stress levels in LPS-treated animals. LPS administration led to increases of ionized calcium-binding adaptor molecule-1 (Iba-1) and glial fibrillary acidic protein (GFAP) expression. Iba-1 and GFAP are well accepted as markers of activated microglia and astrocytes, respectively. Moreover, LPS exposure induced increases of NF-κB and pro-inflammatory factors, such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Increases of these inflammatory mediators by LPS exposure indicate that LPS leads to inflammatory responses and tissue damage. These results demonstrated that LPS activates neuroglial cells and increases NF-κB-mediated inflammatory factors in the cerebral cortex. Thus, these findings suggest that LPS induces neurotoxicity by increasing oxidative stress and activating neuroglia and inflammatory factors in the cerebral cortex.

Hyperglycemia aggravates decrease in alpha-synuclein expression in a middle cerebral artery occlusion model / 한국실험동물학회지

Hyperglycemia is one of the major risk factors for stroke. Hyperglycemia can lead to a more extensive infarct volume, aggravate neuronal damage after cerebral ischemia. α-Synuclein is especially abundant in neuronal tissue, where it underlies the etiopathology of several neurodegenerative diseases. This study investigated whether hyperglycemic conditions regulate the expression of α-synuclein in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury. Male Sprague-Dawley rats were treated with streptozotocin (40 mg/kg) via intraperitoneal injection to induce hyperglycemic conditions. MCAO were performed four weeks after streptozotocin injection to induce focal cerebral ischemia, and cerebral cortex tissues were obtained 24 hours after MCAO. We confirmed that MCAO induced neurological functional deficits and cerebral infarction, and these changes were more extensive in diabetic animals compared to non-diabetic animals. Moreover, we identified a decrease in α-synuclein after MCAO injury. Diabetic animals showed a more serious decrease in α-synuclein than non-diabetic animals. Western blot and reverse-transcription PCR analyses confirmed more extensive decreases in α-synuclein expression in MCAO-injured animals with diabetic condition than these of non-diabetic animals. It is accepted that α-synuclein modulates neuronal cell death and exerts a neuroprotective effect. Thus, the results of this study suggest that hyperglycemic conditions cause more serious brain damage in ischemic brain injuries by decreasing α-synuclein expression.

Association of oral lichen planus with hepatitis-C and diabetes mellitus

The aim of the study is to determine the association of oral lichen planus with hepatitis C and diabetes mellitus

A retrospective cohort study was carried out at the Department of Oral and Maxillofacial Surgery, Sardar Begum Dental College, Peshawar from January 201 1 to July 2015. A total of 34 male and female patients having oral lichen planus were selected with age range 20-60 years and mean age 39.4 +/- 15.8 years. Only clinical features were included to diagnose oral lichen planus which was made through the clinical evaluation of patient's oral cavity by a specialist team at the Department as proposed by van der Meij et al in 2003 based on the WHO definition of oral lichen planus. The data were analyzed through SPSS 22 with significance level ofp-value <0.05 and chi-square statistics were applied for association

The results showed that the data was statistically not significant with p-value >0.05 for all diseases having lichen planus and chi-square statistics revealed that no association exist among lichen planus, diabetes mellitus and hepatitis C. This study fails to find any association between oral lichen planus, hepatitis C and diabetes mellitus

Causes of tooth extraction amongst diabetic patients seen in a dental hospital in Peshawar

The aim of the study was to evaluate the causes of common conditions leading to tooth extraction, male/female ratio in diabetic patients. This was a retrospective cohort study. Data were collected from previous records between January 2011 to July 2015 from the Department of Oral and Maxillofacial Surgery, Sardar Begum Dental College, Peshawar. Among 2,103 total diabetic patients presented to the department, 1,618 diabetic patients have their complete record for tooth extractions. The primary reasons for exodontia specified in the records were Caries, Periodontitis, others. Others include those patients who have extractions either due to preprosthetic purpose, trauma or fracture or any other reason other than caries and periodontitis. The data was analysed through SPSS 22. The mean age presentation was 47.50 +/- 19.82 years and the age range 25-70 years. There were 1,618 extractions carried out in 2,103 subjects [76.94%]. The female diabetic patients predominate n=983 [60.75%] the male patients n=635 [39.25%]. Caries was the dominant cause of tooth extraction in both subjects n= 1042 [64.40%] followed by periodontitis n= 416 [25.71%] and others n=160 [9.90%]. Caries is the dominant cause of extraction in diabetic patients and female diabetic patients predominate male diabetic patients in terms of tooth extraction

Frequency of complete heart block after surgical closure of perimembranous ventricular septal defect

Objective: to find the frequency of complete heart block [CHB] in patients undergoing surgical perimembranous Ventricular septal defect [VSD] closure

Methodology: this was a Descriptive cross sectional study performed in Cardiovascular Department Lady Reading Hospital Peshawar. Data was collected from 28 January 2013 to 28 July 2013 with sample size of 103.Sampling technique was non probability consecutive. All patients with perimembranous ventricular septal defect, aged 5 years to 25 years with any gender were included in the study

Results:-mean age was 12.63 years +/- 6.63. Patients were divided in four categories according to their age. Over all complete heart block in the perimembranous ventricular septal defect after surgical closure was 10[9.71%]. Age wise distribution of complete heart block shows that majority of the complete heart block 6[12.8%] were found in age less than or equal to 10 years

Conclusion:-VSD closure is less often associated with CHB but there should be arrangements for pace maker to timely pace the patient in case of any emergency

Patterns of minor salivary gland tumors in Khyber Pahktunkhwa

Present study was carried out to determine the pattern of minor salivary gland tumors, diagnosed at City Medical Laboratory, Peshawar [Pakistan]. The study was conducted at Sardar Begum Dental College using the Anatomical pathology records of City Medical Laboratory, Peshawar from January 2003 to December 2012 and were analyzed by Statistical Program for Social Sciences version 16. A total of 78 cases were diagnosed as minor salivary gland tumors. Out of these 47 were benign and 31 malignant. The male to female ratio for both benign and malignant tumors was 1:1.7. The mean age was 40.39 years. The most common benign tumor was Pleomorphic adenoma [89.3%] and malignant was Adenoid cystic carcinoma [87.09%]. The hard palate was the most common affected site [57.4% in benign and 32.2% in malignant tumors]. The present study showed a different pattern of occurrence of malignant minor salivary gland tumors among the population of Khyber Pahktunkhwa as compared with the European and American population

Use of mobile phones while driving and resultingfacial injuries

The purpose of this study was to give an overview of drivers use of mobile phones while driving and its role in motor vehicle crashes and subsequent facial injuries. 200 patients who were either drivers or passengers involved in facial injuries as a result of road traffic crashes between January 2008 and November 2008 were included in this study. They were seen in the Department of Oral and Maxillofacial Surgery at Khyber College of Dentistry, Khyber Pakhtunkhwa, Pakistan. All relevant informations were collected on a specially designed Proforma and analysed using SPSS version 17. Male outnumbered female with a male to female ratio of 6:1. Passengers were injured more frequently [60%] as compared to the drivers [40%]. 32 drivers [40%] out of 80 had used mobile phone at the time of accident. Among those drivers [n=32] who had used mobile phone at the time of accident, the 3rd decade was the most commonly involved age group followed by 2nd decade. Mandible was the most common bone involved in 46.88% of the cases followed by Zygomatic complex fractures [21.88%].This study has highlighted the problem of road traffic injuries among drivers and passengers asa serious public health problem. Mobile phone users commit more errors and lapses than non-mobilephone users. It seems that cellular mobile phone bring extra workload to memory and share attention sources, which causes accidents by distracting the attention of drivers

Condylar fractures in children - a study

The purpose of the study was to highlight the etiology, age, gender and site distribution, treatment options available and complications occurring as a result of condylar fractures in growing children. This retrospective study was carried out on a total of 57 patients from Jan 2008 to Oct 2009 at the department of oral and maxillofacial surgery, Khyber College of Dentistry, Peshawar, Khyber Pakhthunkhwa province of Pakistan. Fifty seven patients were recruited in this study. The male to female ratio was 1.6:1. Fall was the most common cause of condylar fractures i.e., [73.7%]. The peak incidence of condylar fracture was high among 6-10 years age group i.e. 26 [45.6%]. Forty one patients [71.9%] had unilateral condylar fractures and [28.1%] had bilateral. Maxillomandibular fixation [MMF] was performed in 28 patients [49.2%] to correct occlusal disturbance, minor deviations and to reduce the pain associated with it, while occlusion was disregarded in 29 patients [50.8%] but were put on close follow up. Overall 41 patients [71.9%] were treated successfully with conservative treatment approach but 16 patients [28.1%] have reported after 1-2 years with complications of temporomandibular joint [TMJ] Ankylosis. Among these 10 patients [62.5%] had unilateral TMJ Ankylosis. Gap arthroplasty with disc interpositioning was performed in patients with established complication of temporomandibular joint ankylosis

Treatment of oroantral fistula - a study

The aim of this study was to evaluate and analyze the occurrence, characteristics and treatment outcome of oroantral fistula in 29 patients from Sept 2004 to Nov 2009. These patients were examined both clinically and radio-logically for oroantral fistula. Data regarding the age, gender, cause and site of fistula and treatment outcome was evaluated and reviewed. The age range was 18-60 years with high frequency occurring in 31-40 years. Male outnumbered female. The common cause of OAF was extraction of teeth [n=25, 86.5%] followed by cysts [n=2, 6.7%]. The most common involved tooth in the causation of OAF was upper first molar [n=13, 52%]. Surgical technique used to close the fistula was buccal advancement flap. Recurrence of fistula occurred in 2 patients [6.7%] and were re-operated using the palatal flap. The merits and demerits of the procedure are discussed

Maxillofacial and associated fractures of the skeleton - a study

The purpose of the present study was to evaluate the incidence of associated skeleton fractures with maxillofacial fractures and to list age gender and bone involved with poly trauma patients. The study is first of its kind in the region and will help to develop a better poly trauma management team. A Descriptive study was carried out in Oral and Maxillofacial Surgery Department of Khyber College of Dentistry, Peshawar, Pakistan for a period of one year i.e., from 20[th] June 2009 to 19[th] June 2010. All the patients presenting with maxillofacial injures and associated fractures of the skeleton were included in this study. Age, gender distribution, aetiology, associated injuries and treatment modalities undertaken in these patients were recorded. A total of 505 patients reported to oral and maxillofacial surgery unit of Khyber College of Dentistry, Peshawar, and amongst them 54 [10.6%] had associated fractures of the rest of the skeleton of the body. The most commonly involved bone was mandible [67%] followed by zygomatic complex fratures i.e. 15% in these patients. 50% of the fractures resulted because of Road Traffic Accidents. Upper limb fractures were the most common bone fractures associated with maxillofacial trauma i.e., 53.25% of the cases

Retaining or extracting a tooth in the line of mandibular fracture - a study

The purpose of the study was to compare the treatment outcome of extractions and nonextractions of teeth in the lines of mandibular fractures and to determine the most common complications. A Quasi-experimental study was undertaken in the Department of Oral and Maxillofacial surgery, Khyber college of Dentistry Peshawar from January 2007 to January 2008. A total of 100 patients having tooth in the line of mandibular fracture were recruited in the study. The patients were divided into two groups of 50 patients each. In Group A the tooth was retained in the line of fracture, and while in Group B the tooth was extracted. Each patient was evaluated for postoperative results and the differences between them assessed. Statistical analysis included chi-square test. Results were considered significant if p d" 0.05. Majority of the patients were young adults. The most common site was parasymphysis while the most commonly involved teeth in the fracture line were incisors. The major operative morbidity proved to be infection followed by malunion and maloclusion. Both groups were not different in relation to postoperative infection [P= 0.461], malocclusion [P= 0.5577], malunion [P= 0.5577] delayed union and non-union. There was no significant difference in the outcome between the extraction and non-extraction of a tooth in the line of fracture

Cancrum oris - a descriptive case series on 13 patients

To evaluate the age, gender, site distribution and associated systemic debelitating conditions [if any] of the patients with Cancrum Oris. A. descriptive retrospective study was undertaken in the Oral and Maxillofacial Surgery unit of Khyber College of Dentistry from January 2005 to January 2010. Information regarding the variables of the study i.e., age, gender, site distribution and associated systemic debilitating conditions, was collected from the ward charts during the study period. A. total of 13 patients reported to the unit during the study period, 8 males and 5 females with male to female ratio of 1.6:1. The age range of the patient was from 9 to 70years with the mean age of 37.69 SD + 22.65. The first and the third decade of life was the most common age group affected with Cancrum Oris i.e., 23.07% each, with another peak in the sixth decade of life i.e., 23.07%. bilateral anterior maxilla was affected in 30.76% cases. Uncontrolled diabetes was the common associated systemic debilitating condition i.e., 72%. All of the patients were from low socio-economic class. Cancrum oris is a disease of low-socioeconomic group. In our series of patients the most common associated debilitating systemic condition was diabetes melitis with a bimodal age distribution

frequency of caries in school going children of Peshawar

To determine the frequency of dental caries, the gender distribution, the most commonly involved deciduous and permanent tooth in dental caries and to evaluate tooth brushing habits in school going children of Peshawar. This descriptive study consisted of 1000 children aged between 6 to 14years old. The information was gathered by taking a detailed history and clinical examination of the students and noted in a structured questionnaire. Brief biodata and oral hygiene levels were recorded in descriptive form while the carious teeth were recorded in tabulated form. Data was analysed using SPSS 10. Out of a 1000 students, 642 [64.2%] had caries. The caries distribution was more in boys [54%] as compared to girls [46%], with male to female ratio 1:0.85. The highest prevalence of caries was found in 12 year old children [77%]. Highest numbers of caries were found in first molar in both primary [262] and permanent dentition [188]. Thirty eight percent children claimed to have brushed their teeth once daily and 11.5% did not brush their teeth at all. Our study showed that there is a lack of awareness of oral hygiene and its importance has not been emphasised enough to the school going children